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Reticulum, the second compartment of the ruminant stomach, is crucial in the stratification and sorting of ingested particles. Early-life nutrition influences forestomach development; however, its specific effects on reticular morphology are not well understood. This study evaluated the impact of two solid feed regimens—starter concentrate (Group A) versus alfalfa hay (Group B)—on reticular development in 60-day-old Holstein calves receiving 8 L/day of milk replacer. Twenty male calves were randomly assigned to the dietary treatments, and feed intake, body weight, and reticular morphometry were assessed. Reticulum samples were examined macroscopically, histologically, and using scanning electron microscopy. No significant differences were found in gross reticulum weight or linear dimensions between groups. Histological analyses, however, revealed greater structural complexity in Group B. Forage-fed calves exhibited more developed primary, secondary, and tertiary reticular crests (cristae reticulares), larger papillae along free margins, and more prominent unguiculiform papillae. These structural features likely improve mechanical sorting and retention of fibrous particles, promoting efficient fermentation. Starter-fed calves showed simpler crest architecture and smaller papillae, consistent with lower fiber intake; however, papillary development was still evident, indicating that solid feed contributes to epithelial growth. These findings emphasize the reticulum as a distinct anatomical and functional unit within the forestomach. Early inclusion of adequate dietary fiber through alfalfa hay enhances reticular complexity, which may support more effective particle sorting, improved fermentation, and long-term digestive efficiency. Overall, pre-weaning feeding strategies that incorporate fiber can positively influence reticular development, with potential benefits for nutrient absorption, digestive function, and calf growth.
References
Antúnez-Tort, G., Bach, A., Cajarville, C., Vidal, M., Fàbregas, F., Ahangarani, M. A., & Terré, M. (2023). Benefits of barley straw as a forage for dairy calves before and after weaning. Journal of Dairy Science, 106(11), 7578-7590. https://doi.org/10.3168/jds.2023-23401. PMid:37558048.
Association of Analytical Communities – AOAC. (2000). Official methods of analysis of AOAC International (17th ed.). AOAC International.
Baldwin, R. L., McLeod, K. R., Klotz, J. L., & Heitmann, R. N. (2004). Rumen development, intestinal growth and hepatic metabolism in the pre- and postweaning ruminant. Journal of Dairy Science, 87(Suppl.), E55-E65. https://doi.org/10.3168/jds.S0022-0302(04)70061-2.
Clauss, M., Hofmann, R. R., Streich, W. J., Fickel, J., & Hummel, J. (2009). Convergence in the macroscopic anatomy of the reticulum in wild ruminant species of different feeding types and a new resulting hypothesis on reticular function. Journal of Zoology (London, England), 281(1), 26-38. https://doi.org/10.1111/j.1469-7998.2009.00675.x.
Drackley, J. K. (2008). Calf nutrition from birth to breeding. The Veterinary Clinics of North America. Food Animal Practice, 24(1), 55-86. https://doi.org/10.1016/j.cvfa.2008.01.001. PMid:18299032.
Dupont, S., Morsut, L., Aragona, M., Enzo, E., Giulitti, S., Cordenonsi, M., Zanconato, F., Le Digabel, J., Forcato, M., Bicciato, S., Elvassore, N., & Piccolo, S. (2011). Role of YAP/TAZ in mechanotransduction. Nature, 474(7350), 179-183. https://doi.org/10.1038/nature10137. PMid:21654799.
Dyce, K. M., Sack, W. O., & Wensing, C. J. G. (2012). Textbook of veterinary anatomy (4th ed.). Saunders.
Hofmann, R. R. (1973). The ruminant stomach. Stomach structure and feeding habits of East African game ruminants (Vol. 2, East African Monographs in Biology). East African Literature Bureau.
Ingber, D. E. (2006). Cellular mechanotransduction: Putting all the pieces together again. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 20(7), 811-827. https://doi.org/10.1096/fj.05-5424rev. PMid:16675838.
Jasper, J., & Weary, D. M. (2002). Effects of ad libitum milk intake on dairy calves. Journal of Dairy Science, 85(11), 3054-3058. https://doi.org/10.3168/jds.S0022-0302(02)74391-9. PMid:12487471.
Langer, P. (1988). The mammalian herbivore stomach: comparative anatomy, function, and evolution. Gustav Fischer.
National Research Council – NRC. (2001). Nutrient requirements of dairy cattle (7th ed.). National Academies Press.
Okine, E. K., Mathison, G. W., Kaske, M., Kennelly, J. J., & Christopherson, R. J. (1998). Current understanding of the role of the reticulum and reticulo-omasal orifice in the control of digesta passage from the ruminoreticulum of sheep and cattle. Canadian Journal of Animal Science, 78(1), 15-21. https://doi.org/10.4141/A97-021.
Sakata, T., & Tamate, H. (1979). Rumen epithelium cell proliferation accelerated by propionate and acetate. Journal of Dairy Science, 62(1), 49-52. https://doi.org/10.3168/jds.S0022-0302(79)83200-2. PMid:457976.
Schummer, A., Klöppel, K., & Rommel, E. (1981). Anatomy of the ruminant stomach. Springer-Verlag.
Teixeira, A. F., Kühnel, W., Vives, P., & Wedel, T. (2009). Functional morphology of unguiculiform papillae of the reticular groove in the ruminant stomach. Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft, 191(5), 469-476. https://doi.org/10.1016/j.aanat.2009.06.005. PMid:19619994.
Terler, G., Velik, M., Poier, G., Sener-Aydemir, A., Klevenhusen, F., & Zebeli, Q. (2023). Feeding concentrate with hay of different qualities modulates rumen histology, development of digestive tract as well as slaughter performance and meat quality of young dairy calves. Archives of Animal Nutrition, 77(3), 171-186. https://doi.org/10.1080/1745039X.2023.2197633. PMid:37255000.
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2. PMid:1660498.
Vazquez, N., Dos Santos, D., Antúnez, G., Amaro, N., Escardó, J., López, L., & Cajarville, C. (2025). Are there differences in abomasal morphology in calves fed eight liters of milk and concentrate or good quality alfalfa hay? Brazilian Journal of Veterinary Medicine, 47, e002825. https://doi.org/10.29374/2527-2179.bjvm002825. PMid:41035544.
Vazquez, N., Dos Santos, D., Artigas, R., Antúnez, G., Amaro, N., López, M., Mass, R., & Cajarville, C. (2026). Does the nature of solid feed influence rumen development? A comparative study of calves fed a high milk replacer with starter concentrate or alfalfa hay. Brazilian Journal of Veterinary Medicine, 48, e009725. https://doi.org/10.29374/2527-2179.bjvm009725. PMid:41567518.
Yohe, T. T., Dennis, T. S., Buss, L. N., Croft, E. J. D., Quigley, J. D., Hill, T. M., Suárez-Mena, F. X., Aragona, K. M., Laarman, A. H., Costa, J. H. C., & Steele, M. A. (2022). Performance and visceral tissue growth and development of Holstein calves fed differing milk replacer allowances and starch concentrations in pelleted starter. Journal of Dairy Science, 105(5), 4099-4115. https://doi.org/10.3168/jds.2021-21286. PMid:35221069.
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Copyright (c) 2026 Noelia Vazquez, Dellis Dos Santos, Germán Antúnez, Rody Artigas, Nicolás Amaro, Flavia Zanolli, Emilia Lanza, Cecilia Cajarville